Insertion junction: CLIP2.062262_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.062262
Chromosome:chromosome 2
Location:8096146
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g144250 CYP769A1,CYP13 (1 of 1) 1.14.13.30 - Leukotriene-B(4) 20-monooxygenase / LTB(4) omega-hydroxylase; Cytochrome P450, CYP4 superfamily 3'UTR
Cre02.g144251 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCACATGCATGACCAGCTGTCTCACACCTACGCTGCTAAGCACACACA
Internal bar code:CGGTTCTTCGGATATAGGAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:574
LEAP-Seq percent confirming:85.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTTAACCCCAACGTGCAG
Suggested primer 2:CCACAATGAGGGCGAAGACT