| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.062296 |
| Chromosome: | chromosome 11 |
| Location: | 1813497 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467550 | (1 of 1) 3.5.2.3 - Dihydroorotase / DHOase; Dihydroorotase/amidohydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCCTATGGAGAAGCCCCCAAACCGCCATGTGCCCAGACAAAAGACGG |
| Internal bar code: | CCATGGAGGGTGCGCGTCACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCGACAAGCTGACTCTG |
| Suggested primer 2: | CCTCCCTTGCCATGTTCCAT |