Insertion junction: CLIP2.062318_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.062318
Chromosome:chromosome 2
Location:2812140
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094450 PHC38,PHC21 Pherophorin-chlamydomonas homolog 38; (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTCAAGGACTAACGTCGGTCTGCCATCTACGCGCGCTGCCTTAAGCAG
Internal bar code:TTGAGCTATAGGGGACGTTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1217
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTAGGGGAACACAGTGG
Suggested primer 2:GCTTGCGCGATCTTGCATTA