| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062328 |
| Chromosome: | chromosome 13 |
| Location: | 5184135 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801588 | (1 of 15) 3.6.5.5 - Dynamin GTPase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCGTAGCGCGCACGCCGGCCTACCCAAGGCCAGTGTGCATGCACCTG |
| Internal bar code: | TATGTGAAGCGAGTATGGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGGCGAAGAAGCTGTGC |
| Suggested primer 2: | CACTGTGTGAAAAGTGCGGG |