| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062335 |
| Chromosome: | chromosome 12 |
| Location: | 2932481 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502400 | (1 of 1) PTHR23423:SF17 - PROTEIN F40E10.6 | 3'UTR | |
| Cre12.g502450 | ELG8 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTACAAGAGTGGAGCCAGGACTGATGAGGGGGGGGCAAGACCTTCAGGC |
| Internal bar code: | CAATAACTCGTGTTCGAGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3712 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTATCTTGGTGTGCCAT |
| Suggested primer 2: | GCTGTGCTCCATGAGTCTGT |