| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062342 |
| Chromosome: | chromosome 10 |
| Location: | 3838851 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445900 | (1 of 1) PF00226//PF14901 - DnaJ domain (DnaJ) // Cleavage inducing molecular chaperone (Jiv90) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACCCCATGTCCCAAAACCTCCCGACCCCCCCTCCGGACCGACCGACCG |
| Internal bar code: | GAGTGGTAAGTGCCAATAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1373 |
| LEAP-Seq percent confirming: | 49.0909 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCGGTGGGTGAAGATG |
| Suggested primer 2: | GCGCTCACAGCCACATAATG |