| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062350 |
| Chromosome: | chromosome 1 |
| Location: | 2950447 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018500 | HAS1 | (1 of 1) PTHR12215//PTHR12215:SF10 - PHOSPHOPANTETHEINE TRANSFERASE // L-AMINOADIPATE-SEMIALDEHYDE DEHYDROGENASE-PHOSPHOPANTETHEINYL TRANSFERASE; Holo-[acyl-carrier-protein] synthase | 3'UTR |
| Cre01.g018550 | CYG69 | (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTAGAGGCGCGCGCTCCACCGGCGTGTGCCCGCTGGAGGCGGCTTCAC |
| Internal bar code: | TCACTTTGGGGACCCCTGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4228 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTAACTCTTGCGGCAGTGG |
| Suggested primer 2: | GTGGTGACAACAGCGACAAC |