Insertion junction: CLIP2.062361_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062361
Chromosome:chromosome 7
Location:5476524
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g351050 VPS23 (1 of 1) K12183 - ESCRT-I complex subunit TSG101 (TSG101, STP22, VPS23); Subunit of the ESCRT-I complex 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATAGCCTAGAGCAAGGCTAGGTGGTAGGCATCTTCTCGCCCTAGTTCAT
Internal bar code:GAGCGATCTTTGTGTTTGTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2567
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CATACCGTCCCTCACTACGC
Suggested primer 2:GGCCGGATGATCATGTTTGC