| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.062368 |
| Chromosome: | chromosome 3 |
| Location: | 1293283 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150151 | NRT2E | Nitrate/nitrite transporter; (1 of 5) PTHR23515:SF2 - HIGH AFFINITY NITRATE TRANSPORTER 2.3-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGGCAGACGCCGGTCTGGCGCCACGCCGTGGAGGCGATGCCACGTGG |
| Internal bar code: | CAAATGGTTACACGGTAAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2781 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAATGGAGAGTCCCACAA |
| Suggested primer 2: | TCACTGTCGATGGCAATGCT |