| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062370 |
| Chromosome: | chromosome 10 |
| Location: | 5723064 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459600 | (1 of 1) K14832 - ribosome biogenesis protein MAK21 (MAK21, NOC1, CEBPZ) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATTAATCTTCCCTTTAGCACATGTTCTAGGCGACACTGCACGGGCGCC |
| Internal bar code: | TCTCGCATACCCATGCGAGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4523 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTGAGCCCCTTGGACTC |
| Suggested primer 2: | TAAGCGGAGGCATTCTTGGG |