| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062381 |
| Chromosome: | chromosome 8 |
| Location: | 2623255 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373250 | POA2 | 20S proteasome alpha subunit B; (1 of 1) K02726 - 20S proteasome subunit alpha 2 (PSMA2) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGAGTCCCCGCGCCGATCCCGCCCGATCCCGCCCATTCGGCCCACCGT |
| Internal bar code: | GTTTATATGAGTATCCACGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3555 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACACCACACCGATCTGC |
| Suggested primer 2: | CGCCTTGAAAACCTTTCGCA |