Insertion junction: CLIP2.062398_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.062398
Chromosome:plastome
Location:135669
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802320 chlN,2716986,ChreCp056 light-independent protochlorophyllide reductase subunit N; (1 of 1) K04038 - light-independent protochlorophyllide reductase subunit N (chlN) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTTCGGCAAAAATCATAACTCCAAGGGCATTTTGTAAAAAATAACCAC
Internal bar code:TTGGGTGATCGTGCGATAGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:438
LEAP-Seq percent confirming:63.3333
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:AGATGGGTGTAGCATCTCGC
Suggested primer 2:CCATCGGGGCCTATTGGAAA