Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.062398 |
Chromosome: | plastome |
Location: | 135669 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
CreCp.g802320 | chlN,2716986,ChreCp056 | light-independent protochlorophyllide reductase subunit N; (1 of 1) K04038 - light-independent protochlorophyllide reductase subunit N (chlN) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTCGGCAAAAATCATAACTCCAAGGGCATTTTGTAAAAAATAACCAC |
Internal bar code: | TTGGGTGATCGTGCGATAGAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 438 |
LEAP-Seq percent confirming: | 63.3333 |
LEAP-Seq n confirming: | 19 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGATGGGTGTAGCATCTCGC |
Suggested primer 2: | CCATCGGGGCCTATTGGAAA |