Insertion junction: CLIP2.062401_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062401
Chromosome:chromosome 17
Location:526522
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g699350 SMM51 (1 of 13) PF12847 - Methyltransferase domain (Methyltransf_18); S-adenosyl-L-methionine-dependent methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGCGCGTGTATGCCCTCCGCTCTCACACACTTGGGCCGCTGGGTACGG
Internal bar code:AAAGGATGAGGCTTTAAATAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1745
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCACTTCATTGAGCTGCACC
Suggested primer 2:CTGCCTTGACTGCCTGTACA