| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062407 |
| Chromosome: | chromosome 9 |
| Location: | 3551393 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396587 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGACCGCTGACTATTACCGCCGCTTGACTTGCCGTGGCGGTGTGGGCG |
| Internal bar code: | CAGGAGGAGCACTGTTCTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2583 |
| LEAP-Seq percent confirming: | 16.9014 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGGTGGATGAGGAGGT |
| Suggested primer 2: | CGGCCAGAAGTTCACCTCAT |