Insertion junction: CLIP2.062408_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.062408
Chromosome:chromosome 3
Location:4117427
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g172600 (1 of 1) IPR000104//IPR001841//IPR011990//IPR013083 - Antifreeze protein, type I // Zinc finger, RING-type // Tetratricopeptide-like helical domain // Zinc finger, RING/FYVE/PHD-type 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTCCTGCCCGCTGGGCTTCGGCGGGCCGGCTTCAAAGAGCTCGCTGAC
Internal bar code:TGGATCTGAGGTTTGTTCTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:698
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTGATGTTGGTGCGGAGGT
Suggested primer 2:AAGTAGTAGTCGGCGGAGGT