| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062411 |
| Chromosome: | chromosome 12 |
| Location: | 6237178 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGCGGGTGGTGGCGCCATGGTGGCGGCGGCCTCCTGTACACTCTGCA |
| Internal bar code: | TGCGTTTTCGTTATAATGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5149 |
| LEAP-Seq percent confirming: | 97.8022 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAAGGGTTTGCTGCAAC |
| Suggested primer 2: | GGCAGCCACCTCCTTGTTAT |