Insertion junction: CLIP2.062420_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062420
Chromosome:chromosome 12
Location:5235640
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g527500 (1 of 1) K04508 - transducin (beta)-like 1 (TBL1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCGGCGTCCCCACACGCGAACAATGTGTTGAGCTTGCGACTAGGACAG
Internal bar code:TCAGGATCACGACTCCACACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:4427
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:ACATGGCAGCGCTTTTCAAG
Suggested primer 2:CGTCACAGTGTACCTGCAGT