Insertion junction: CLIP2.062422_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.062422
Chromosome:chromosome 12
Location:6270947
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g536450 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAACCCCGACTTTGCTGCAAACCCTCCCGCGGGCAAAGTCCGTGTGAC
Internal bar code:AGGTTCAACGTAGGCAGTTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1643
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTTTTGGGACTGCACTGT
Suggested primer 2:CGGTCTGCCCTTTGAGCTTA