Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.062422 |
Chromosome: | chromosome 12 |
Location: | 8306357 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g551100 | (1 of 1) PTHR36356:SF1 - EXPRESSED PROTEIN | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGAGCCACGGCCGCGGCCGCCGCCGCCACCCGAGCTGGCGGCGGCGCC |
Internal bar code: | GGTCAGTGTACTGCGGTCTAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 5839 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 27 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGGTAGTGGATCCCATGGA |
Suggested primer 2: | GTCGTGCACACTCTACACCA |