| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062426 |
| Chromosome: | chromosome 17 |
| Location: | 917101 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g702750 | SDR27 | (1 of 1) 1.1.1.102 - 3-dehydrosphinganine reductase / KTS reductase; Short-chain dehydrogenase/reductase | 5'UTR |
| Cre17.g702800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTATGCTTATCAAAGAGCTAGGTGGCCGATTTGCGCTCTCATCAACGCT |
| Internal bar code: | GATTTTGGTTGAGTGCCTCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 928 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAAGAAGGATCGGGGCT |
| Suggested primer 2: | GGTAACCAAGTGCCGTCAGA |