| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062427 |
| Chromosome: | chromosome 16 |
| Location: | 3094268 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664700 | SHY1 | (1 of 1) K14998 - surfeit locus 1 family protein (SURF1, SHY1); Cytochrome c oxidase assembly protein, Surfeit 1-like protein | 3'UTR |
| lncRNA_TCONS_00223190 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAACGGCACTTGTCGCTCCAAACCGCTACATGCCAAGATGGCGCCTA |
| Internal bar code: | CGTTTTAAGGGGACGGTTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4296 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCGTCCGGTCCTATCGAT |
| Suggested primer 2: | CACGTTCTGGAGTGCAGCTA |