Insertion junction: CLIP2.062451_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.062451
Chromosome:chromosome 4
Location:2562458
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g222550 (1 of 43) IPR011989 - Armadillo-like helical 3'UTR
Cre04.g222600 PHC46 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCAAGCCTTTCCAAACATGCTACTTTGGCAGACGCGACCTCCGTACG
Internal bar code:CGCTTTTCACAATATGGACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:487
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGTGCTTTACGCTGAGT
Suggested primer 2:TCCAGGCTGACATTGACACC