Insertion junction: CLIP2.062451_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.062451
Chromosome:chromosome 4
Location:2562459
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g222550 (1 of 43) IPR011989 - Armadillo-like helical 3'UTR
Cre04.g222600 PHC46 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGTTGGATGTCGCGGTGGCAGGATCGCGTGAACGATAAGAGGCCTCAA
Internal bar code:CGCTTTTCACAATATGGACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:906
LEAP-Seq percent confirming:78.7879
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TCCAGGCTGACATTGACACC
Suggested primer 2:AGCTGTGCTTTACGCTGAGT