| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062465 |
| Chromosome: | chromosome 2 |
| Location: | 8298643 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143250 | IDH2 | (1 of 2) 1.1.1.41 - Isocitrate dehydrogenase (NAD(+)) / Nicotinamide adenine dinucleotide isocitrate dehydrogenase; Isocitrate dehydrogenase, NAD-dependent | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGTGTTTGGGTTTTCCCGAGTGCACAATCGCGTGCCCCATGCGGTCG |
| Internal bar code: | TCCGTAGTGACAGGTAGCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1487 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAAATCCGATCTAGCGA |
| Suggested primer 2: | GCGTAAAGGTCCAGCTCCTT |