| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062467 |
| Chromosome: | chromosome 2 |
| Location: | 86212 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g073650 | PRP19 | Spliceosome Component, nuclear pre-mRNA splicing factor; (1 of 1) K10599 - pre-mRNA-processing factor 19 (PRPF19, PRP19) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCTGATAGCTCTCGTGCCCTGCCCTATTCCCATCGCAGCTGGCGACC |
| Internal bar code: | AAGCTAAGTAAGGGAAGCGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4643 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTTGATAGGTGCTTGGT |
| Suggested primer 2: | GCGAACCCTTGAGTTAGGCT |