| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062482 |
| Chromosome: | chromosome 16 |
| Location: | 276317 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694250 | (1 of 18) PF12937 - F-box-like (F-box-like) | 5'UTR | |
| Cre16.g694300 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTAATGCTATCACCGAAATAACCAAGTGACACGCGGCTACTTCTCTGC |
| Internal bar code: | GTCATAGATAGTGGGTACGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2117 |
| LEAP-Seq percent confirming: | 76.4706 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACGCATACACGCACATG |
| Suggested primer 2: | ATTTGCAACGCGTGAGTGAC |