| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.062508 |
| Chromosome: | chromosome 3 |
| Location: | 8010002 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCGTGAAGGCACGCACCAGCTGTAAACGCAACAGAATGCCTTGAGCA |
| Internal bar code: | TGTTGTCGTTGCTCACTTATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2041 |
| LEAP-Seq percent confirming: | 91.1111 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACTCAAGCCGCAGACTG |
| Suggested primer 2: | GATGGCTGCGTAGGGTACTC |