| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.062522 |
| Chromosome: | chromosome 7 |
| Location: | 4764488 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g345750 | NAT20 | N-acetyltransferase; (1 of 3) IPR000182//IPR011011//IPR016181 - GNAT domain // Zinc finger, FYVE/PHD-type // Acyl-CoA N-acyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCAACTCGCACAAATCATATCTCATGGTAGAAGACACGTATTCATCA |
| Internal bar code: | CATGCAACAATAAGGGTAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1435 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGTCCACACCACCCATT |
| Suggested primer 2: | CCAAGCGTCTTTTTCCCAGC |