| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.062523 |
| Chromosome: | chromosome 8 |
| Location: | 4173003 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383650 | NUP85 | Nucleoporin 85; (1 of 1) K14304 - nuclear pore complex protein Nup85 (NUP85) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTAATTACATAGTTTTGAAAGACGATACAACTCACTGGAGTGCAGAGC |
| Internal bar code: | ATGAACGTGTTTCATTGTTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCGTGTTCGTGGTTTGTG |
| Suggested primer 2: | GCATCCACTCCACCCATTCA |