| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062531 |
| Chromosome: | chromosome 6 |
| Location: | 1990472 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264900 | HTB13 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAATCGCACTGAATGACAATGCGCTATTTATTGCTGGAGTCGGCATCGG |
| Internal bar code: | CTATGCGTGTGGCAGGCACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3872 |
| LEAP-Seq percent confirming: | 82.1782 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 101 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGAGTTTGAGGGAAGCAG |
| Suggested primer 2: | TTACGTCCCGTGTCAAGGTG |