| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.062531 |
| Chromosome: | chromosome 6 |
| Location: | 1990475 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264900 | HTB13 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCCCAAGACACTCAGTACCTCCGCCGCATTAACAAGTCTATCAATATG |
| Internal bar code: | CTATGCGTGTGGCAGGCACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1640 |
| LEAP-Seq percent confirming: | 65.7895 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACGTCCCGTGTCAAGGTG |
| Suggested primer 2: | ACCGAGTTTGAGGGAAGCAG |