| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.062533 |
| Chromosome: | chromosome 4 |
| Location: | 695713 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217953 | (1 of 2) PTHR15241:SF0 - TRANSFORMER-2 SEX-DETERMINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTGGGCTCCAAGAGCAGCGTCGCAGAGCCCGCCAACCGGCGCCGTAA |
| Internal bar code: | CGGGAATCTACCATTATAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 792 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATCAGGCCAAACATCCT |
| Suggested primer 2: | TGAAGAGGCAGGCACAGAAG |