Insertion junction: CLIP2.062549_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.062549
Chromosome:chromosome 6
Location:3623139
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278138 OPR119 OctotricoPeptide Repeat protein 119, pseudogene; (1 of 781) IPR000104 - Antifreeze protein, type I 3'UTR
lncRNA_TCONS_00179413 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTACTGACAAGCAGACAACTGTGTAAACCCAAGATTCCAAACTGTGCA
Internal bar code:CCTGTACACGTCCAAGGGGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1671
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:ATACAGCGCGAGTACTGAGC
Suggested primer 2:CCAATCACCACCACCATCCA