| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062554 |
| Chromosome: | chromosome 1 |
| Location: | 5765067 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041300 | ALG10 | a-1%252C2-glucosyltransferase; (1 of 1) 2.4.1.256 - Dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2- glucosyltransferase / Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCCTCGAAGCCCCACCAACGGCCAAACCTGCAGCACCCACCAACCAC |
| Internal bar code: | GGTGGTCAGGAAAGATGAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2073 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATTGCACTTTGCCTGGCC |
| Suggested primer 2: | CCACTGTTCGTTGGCAGTTG |