Insertion junction: CLIP2.062557_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.062557
Chromosome:chromosome 3
Location:8890425
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g205361 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTTCAGCGCCGTGCGCGTGCCCCATCCCGCAAGCCTACCTAACTCCCG
Internal bar code:GTGAAAGACGGGACATATGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3145
LEAP-Seq percent confirming:69.4118
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:85

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCACACACACACACACAC
Suggested primer 2:TTTCCCACGGTGTCGAAGAG