| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.062558 |
| Chromosome: | chromosome 3 |
| Location: | 4095680 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172300 | MPC1,PHT3A,MPC5,CR057,PHT3-1,MCP1 | Mitochondrial phosphate carrier protein; (1 of 1) K15102 - solute carrier family 25 (mitochondrial phosphate transporter), member 3 (SLC25A3, PHC, PIC) | 3'UTR |
| Cre03.g172322 | (1 of 3) PTHR15439//PTHR15439:SF4 - RETINOBLASTOMA-BINDING PROTEIN 6 // ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 24 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGAGCACCGCTGGCTCGCTCTGTGCCGTGAACTCTTGGACTCGGTA |
| Internal bar code: | GAGGTTGGACTTATGGTAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3090 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCTGCTGCCCTAAAGAG |
| Suggested primer 2: | TTCGCCTTGGTTGTGCATTG |