| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062560 |
| Chromosome: | chromosome 10 |
| Location: | 6708546 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466750 | SAT1 | (1 of 1) PTHR23416//PTHR23416:SF2 - SIALIC ACID SYNTHASE-RELATED // SERINE ACETYLTRANSFERASE 5; Serine O-acetyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACGTCTCCGCCGCCAACGTGATCCGTGTGCTCCTCCTGCTGAAGCTGA |
| Internal bar code: | CCCTGGGACGTGTTTAATTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2070 |
| LEAP-Seq percent confirming: | 74.4186 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCGAGGATGACTGGATG |
| Suggested primer 2: | GCATTGTGTTGCGGAGTGTT |