Insertion junction: CLIP2.062592_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.062592
Chromosome:chromosome 5
Location:1156840
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g248550 (1 of 1) K16276 - zinc finger protein-like protein (K16276, BTS) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACTGGGCACTCACGCCTGCTCATCACGTTTTTCTACTTATTGCCACG
Internal bar code:TTCATATAGTCGCGTCCGACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1536
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:AATGTGTGTGTTTGCGGACG
Suggested primer 2:TGGTGGATGGAGGAGGAGAG