| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.062594 |
| Chromosome: | chromosome 12 |
| Location: | 5589158 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531100 | PBA2 | 20S proteasome beta subunit A2; (1 of 2) K02738 - 20S proteasome subunit beta 1 (PSMB6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTCGGCGCAGGCATCTGGACTGCCGCGGCGACCCGCCCCAGGCAGCT |
| Internal bar code: | CCAGCTAATATTGGTTCTTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4465 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAACGGTAAGAATCGGGC |
| Suggested primer 2: | CGTGCCGAGTTTAGATTGCG |