Insertion junction: CLIP2.062601_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062601
Chromosome:chromosome 17
Location:523710
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g699350 SMM51 (1 of 13) PF12847 - Methyltransferase domain (Methyltransf_18); S-adenosyl-L-methionine-dependent methyltransferase 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTAGTGGTAGGCATAAAAACCCAGCGTTCAGCAGGACCAGCTGGGGTAG
Internal bar code:TTTTTAAGATAGGAAACCAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:4442
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTAGCCATCAGTCCGAT
Suggested primer 2:AGTGGGTGTACTTGGCGATG