| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062615 |
| Chromosome: | chromosome 12 |
| Location: | 6622940 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g539000 | ECT1 | (1 of 1) 2.7.7.14 - Ethanolamine-phosphate cytidylyltransferase / Phosphorylethanolamine transferase; CDP-Ethanolamine synthase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACACCCACTCCGACATCAGTGTCTAACTTGCTTTTGTTGGCAGCGGGA |
| Internal bar code: | TTATACCGCTCCGTCGCATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1909 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGAACATAGCATCTCGA |
| Suggested primer 2: | AACGGCATATGCGACAATGC |