| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.062645 |
| Chromosome: | chromosome 16 |
| Location: | 5957563 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678850 | (1 of 1) 2.3.3.10 - Hydroxymethylglutaryl-CoA synthase / Hydroxymethylglutaryl coenzyme alpha-condensing enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGAAGACTACGGAAGTGCTGCTTCATGAAAGACGAACTGCGTACTGT |
| Internal bar code: | CATGAGGCGATCAACTAGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1962 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGTTGGGCCCTGATAGCA |
| Suggested primer 2: | AGCAATGGGAGCAGCAGTAG |