| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062645 |
| Chromosome: | chromosome 16 |
| Location: | 5957569 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678850 | (1 of 1) 2.3.3.10 - Hydroxymethylglutaryl-CoA synthase / Hydroxymethylglutaryl coenzyme alpha-condensing enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGAACCAGTGAAGCCGCCGCACAACAACACACGCGCCATTCGCGAAC |
| Internal bar code: | CATGAGGCGATCAACTAGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4828 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAATGGGAGCAGCAGTAG |
| Suggested primer 2: | CAAGTTGGGCCCTGATAGCA |