Insertion junction: CLIP2.062649_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.062649
Chromosome:chromosome 13
Location:4489755
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre13.g602901 CNX2 (1 of 1) PTHR22960:SF0 - MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1; Cofactor of nitrate reductase and xanthine dehydrogenase 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGCCTGCCCGCTCCCCTCCCCGTTGCCCTCCCCGCCCCCTTTGGTTTC
Internal bar code:GTTTATTACTTCAATAGCGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:68
LEAP-Seq percent confirming:7.69231
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTAGCCTTAAACCCCGTG
Suggested primer 2:CGTAGGGGGTTGCAAGGATT