| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062652 |
| Chromosome: | chromosome 12 |
| Location: | 1290625 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGACATCAAGAGGCACGCATATCAGGCAGGTGGAGACATCTTGCTGA |
| Internal bar code: | AATAGGATCCCATTGGATTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4131 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATGTGCTGCTTCAAGAG |
| Suggested primer 2: | CCAGCTAGGCAATGGGTGAA |