| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.062654 |
| Chromosome: | chromosome 17 |
| Location: | 880043 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g702400 | PUS5 | (1 of 1) 3.5.4.26//5.4.99.28 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase // tRNA pseudouridine(32) synthase / Pseudouridine synthase RluA; RNA pseudouridine synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGCCGCTACGTGCGGGCATGTGCATCCGGCACTTCATCCACAGGCAC |
| Internal bar code: | TGTTACACGAAGTCACAGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGGCTCTACACACAGGT |
| Suggested primer 2: | ATCCCTGACTCTCTCGTGCT |