Insertion junction: CLIP2.062659_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062659
Chromosome:chromosome 2
Location:1214176
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g081650 (1 of 1) PTHR31515//PTHR31515:SF3 - FAMILY NOT NAMED // GENOMIC DNA, CHROMOSOME 3, BAC CLONE: T19N8 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAACACGGCCACCTTCAAAACGGATTGCGCACACTTGGACCCAACACA
Internal bar code:CGCAGCTTGTAAAACATCGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1644
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTATGGCCTCGATACCCGTG
Suggested primer 2:GCTCCAGTTTGTCGTGGGTA