| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062664 |
| Chromosome: | chromosome 12 |
| Location: | 1768904 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACAAAATAGTTGCTGCGCATGTGGGCGCTTGTACTGTGACTTTAAAAC |
| Internal bar code: | TGGGATTTGAATGTAGGCCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1820 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCCAGGGAATATTGCTTG |
| Suggested primer 2: | GCGAGAGGTTCAAGTGGGAA |