| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.062669 |
| Chromosome: | chromosome 13 |
| Location: | 1855693 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575250 | (1 of 1) PTHR10663:SF114 - PROTEIN MON2 HOMOLOG | 5'UTR | |
| Cre13.g575300 | (1 of 1) K08876 - SCY1-like protein 1 (SCYL1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCGTGCTTGTACCCCCGATGGGGCCAAAACTTCAACCACCATCGGCC |
| Internal bar code: | TGCTTAATAAGAGAAACCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3757 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTCTACAACGGCATGCT |
| Suggested primer 2: | CCCCTGTATGGAAACTGCGT |