| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.062670 |
| Chromosome: | chromosome 7 |
| Location: | 5502852 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351350 | CSG8 | Chlamydomonas specific family G protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGCACGTACTTTTAGTTTTTTAAGGGGGTGTCTGGTGAGGAAGTTCT |
| Internal bar code: | CACGATTGACTGGGAGAGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1505 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAAAGTGCCAGACCCCA |
| Suggested primer 2: | ATTGGTCACAACGTTTGCCG |